The field of pharmacology and toxicology is vital in the development of safe and effective pharmaceuticals. The advanced course "Pharmacological and Toxicological Screening Methods-II" designed for the M. Pharm. Pharmacology, Semester-II as per the Curriculum of the Pharmacy Council of India (PCI), serves as a comprehensive guide to understanding the principles and practices essential for drug safety evaluation. This book provides a detailed exploration of various toxicological screening methods, adhering to the latest regulatory guidelines set by organizations such as the OECD, ICH, EPA, and Indian regulatory authorities. The content emphasizes the historical evolution, the significance of Good Laboratory Practices (GLP), and their application in contemporary drug development processes. Covering a wide range of topics, from acute to chronic toxicity studies, reproductive toxicology, genotoxicity, and in vivo carcinogenicity, this book delves into the critical areas that every pharmacologist and toxicologist must master. It also addresses the nuances of IND enabling studies and safety pharmacology, highlighting the need for stringent safety evaluations before clinical trials. The text is structured to facilitate both learning and practical application, making it an indispensable resource for postgraduate students specializing in pharmacology. It provides a solid foundation for understanding the toxicokinetic evaluations and alternative methods to animal testing, aligning with global trends in reducing animal use in scientific research.